Biochemistry


Alan was a Professor of Biochemistry (now retired) at the University of Southern California, Los Angeles. His special areas of interest are in dental enamel proteins and in the use of problem-based learning in dental education.

Dental enamel proteins are the proteins which, during tooth enamel formation, are secreted by specialized cells (ameloblasts) of the tooth bud and define the space which eventually becomes dental enamel. In ways, still not fully understood, these proteins form an extracellular matrix which both induces the mineralization of the structure with crystals of carbonated calcium hydroxyapatite and also appears to organize the orientation and spacing of these crystals at the ultrastructural level.

Dental Enamel Proteins - selected publications

Wen B, Moradian-Oldak J, Zhong JP, Greenspan DC & Fincham AG (2000) Effects of amelogenin on the transforming surface microstructures of Bioglass in a calcifying solution, Journal of Biomedical Materials Research, 52:

Moradian-Oldak J, Paine ML, Lei YP, Fincham AG & Snead ML. Self-assembly properties of recombinant engineered amelogenin proteins analyzed by dynamic light scattering and atomic force microscopy, Journal of Structural Biology, 131: 27-37.

Fincham AG, Luo W, Moradian-Oldak J, Paine ML, Snead ML & Zeichner-David M. (2000) Enamel biomineralization: the assembly and disassembly of the protein extracellular organic matrix, In: (Ferguson MWJ, Teaford M & Meredith-Smith, Eds), Development, Function and Evolution of Teeth, Cambridge University Press, pp. 37-61.

Ravindranath RMH, Tam W-Y, Nguyen P & Fincham AG (2000) Amelogenin proteins bind to a GlcNAc-mimicking peptide motif of cytokeratins, Journal of Biological Chemistry, September 8th

Fincham AG, Moradian-Oldak J & Simmer JP. (1999) The structural biology of the developing dental enamel matrix, Journal of Structural Biology, 126, 270-299.

Hunter GK, Curtis HA, Grynpas MD, Simmer JP & Fincham AG. (1999) Effects of recombinant amelogenin on hydroxyapatite formation in vitro, Calcified Tissues International, 65, 226-231.

Wen HB, Moradian-Oldak J & Fincham AG. (1999) Modulation of apatite crystal growth on Bioglass by recombinant amelogenin, Biomaterials, 20, 1717-1725.

Wen HB, Moradian-Oldak J, Leung W, Bringas PJ & Fincham AG. (1999) Microstructures of an amelogenin gel matrix, Journal of Structural Biology, 126: 42-51.1.

Wen HB, Fincham AG,Moradian-Oldak J & de Groot K. (1999) Crystal growth of calcium phosphate on biomaterials, Recent Research Devel. Crystal Growth Res., 1: 51-65.

Fincham AG (Guest Editor). (1998) Proceedings of the Sixth International Symposium on the Composition, Properties and Fundamental Structure of Tooth Enamel, Lake Arrowhead, Ca. May 11th - 15th. Connective Tissue Research, 38/39, 536pp.

Fincham AG, Leung W, Tan J & Moradian-Oldak J. (1998) Does amelogenin nanosphere assembly proceed through intermediary-sized structures? Connective Tissue Research, 38, 237-240.

Tan J, Leung W, Moradian-Oldak J, Zeichner-David M & Fincham AG. (1998) The pH dependent amelogenin solubility and its biological significance, Connective Tissue Research, 38, 215-221.

Moradian-Oldak J, Leung W, Tan J & Fincham AG. (1998) Effect of apatite crystals on the activity of amelogenin degrading enzymes in vitro, Connective Tissue Research, 38, 435-444.

Lyaruu DM, Hu CC, Zhang C, Qian Q, Ryu OH, Moradian-Oldak J, Woltgens JH, Fincham AG & Simmer JP. (1998) Derived protein and cDNA sequences of hamster amelogenin, European Journal of Oral Sciences, 106, 299-307.

Tan J, Leung W, Moradian-Oldak J, Zeichner-David M & Fincham AG. (1998) Quantitative analysis of amelogenin solubility, Journal of Dental Research, 77, 1388-1396.

Moradian-Oldak J, Leung W & Fincham AG. (1998) Temperature and pH-dependent supramolecular self-assembly of amelogenin molecules: a dynamic light-scattering analysis, Journal of Structural Biology, 122, 320-327.

Moradian-Oldak J, Tan J & Fincham AG. (1998) Interaction of amelogenin with hydroxyapatite crystals: an adherence effect through amelogenin molecular self-association, Biopolymers, 46, 225-238..

Fincham AG & Simmer JP. (1997) Amelogenin proteins of developing dental enamel, Ciba Foundation Symposium Series, 205, 118-130.

Fincham AG & Moradian-Oldak J. (1996) Comparative mass spectrometric analyses of enamel matrix proteins from five species suggest a common pathway of post-secretory proteolytic processing, Connective Tissue Research, 35, 205-210.

Moradian-Oldak J, Leung W, Simmer JP, Zeichner-David M & Fincham AG. (1996) Identification of a novel proteinase (Ameloprotease-I) responsible for complete degradation of amelogenin during enamel formation. Biochemical Journal, 318, 1015-1021.

Moradian-Oldak J, Sarte PE & Fincham AG. (1996) Description of two classes of proteinases from enamel extracellular matrix cleaving a recombinant amelogenin, Connective Tissue Research, 35, 231-238.

Fincham AG, Moradian-Oldak J, Diekwisch TGH, Lyaruu DM, Wright T, Bringas P. & Slavkin HC. (1995) Evidence for amelogenin "Nanospheres" as functional components of secretory-stage enamel matrix, Journal of Structural Biology, 115, 50-59.

Simmer JP & Fincham AG. (1995) Molecular mechanisms of dental enamel formation, Critical Reviews in Oral Biology & Medicine, 6, 84-108.

Moradian-Oldak J, Lau EC, Diekwisch T, Slavkin HC & Fincham AG. (1995) A review of the aggregation properties of a recombinant amelogenin, Connective Tissue Research, 31, 1- 6.

Fincham AG & Moradian-Oldak J. (1995) Recent advances in amelogenin biochemistry, Connective Tissue Research, 32, 119-124.

Fincham AG, Moradian-Oldak J. & Sarte PE. (1994) Mass-spectrographic analysis of a porcine amelogenin identifies a single phosphorylated locus, Calcified Tissue International, 55, 398-400.

Simmer JP, Hu C, Lau EC, Sarte PE, Moradian-Oldak J, Slavkin HC & Fincham AG. (1994) Alternative splicing of the mouse amelogenin primary RNA transcript, Calcified Tissue International, 55, 302-310.

Simmer JP, Lau EC, Hu CC, Bringas P, Santos V, Aoba T, Lacey M, Nelson D, Zeichner-David M, Snead ML, Slavkin HC & Fincham AG. (1994) Isolation and characterization of a mouse amelogenin expressed in Escherichia coli., Calcified Tissue International, 54, 312-319.

Fincham AG, Moradian-Oldak J, Simmer JP, Sarte PE, Lau EC, Diekwisch T & Slavkin HC. (1994) Self-assembly of a recombinant amelogenin protein generates supramolecular structures, Journal of Structural Biology, 112, 103-109.

Moradian-Oldak J, Simmer PJ, Lau EC, Sarte PE, Slavkin HC & Fincham AG. (1994) Detection of monodisperse aggregates of a recombinant amelogenin by dynamic light scattering, Biopolymers, 34, 1339-1347.

Moradian-Oldak J, Simmer PJ, Sarte PE, Zeichner-David M & Fincham AG. (1994) Specific cleavage of a recombinant murine amelogenin by a protease fraction isolated from bovine tooth enamel, Archives of oral Biology, 39, 647-656.

Fincham AG & Moradian-Oldak J. (1993) Amelogenin post-translational modifications: Carboxyterminal processing and the phosphorylation of bovine and porcine "TRAP" and "LRAP" amelogenins, Biochemical and Biophysical Research Communications, 197, 248-255.

Lau EC, Simmer JP, Bringas P, Hsu DJ, Hu CC, Zeichner-David M, Thiemann F, Snead ML, Slavkin HC & Fincham AG. (1992) Alternative splicing of mouse amelogenin primary RNA transcript contributes to amelogenin heterogeneity, Biochemical and Biophysical Research Communications, 188, 1253-1260.

Fincham AG, Hu Y, Lau EC, Slavkin HC & Snead ML. (1991) Amelogenin post-secretory processing during biomineralization in the postnatal mouse molar tooth, Archive of oral Biology, 36, 305-317.

Fincham AG, Bessem C, Lau EC, Pavlova Z, Shuler C, Slavkin HC & Snead ML (1991) Human developing enamel proteins exhibit a sex-linked dimorphism, Calcified Tissue International, 48, 288-290.

Fincham AG, Hu Y, Lau EC, Pavlova Z, Slavkin HC & Snead ML. (1990) Isolation and partial characterization of a human amelogenin from a single fetal dentition using HPLC techniques, Calcified Tissue International, 47, 105-111.

Fincham AG, Bessem C, Bringas P, Hu Y, Snead ML & Slavkin HC. (1989) Amelogenesis in vitro: a model for studies of post-secretory processing during enamel biomineralization, Differentiation, 41, 62-71

Fincham AG, Hu Y, Pavlova Z, Slavkin HC & Snead ML. (1989) Human amelogenins: sequences of "TRAP" molecules, Calcified Tissue International, 45, 243-250

Robinson C, Kirkham J & Fincham AG. (1989) The Enamelin - Non-Amelogenin problem, A brief review. Connective Tissue Research, 22, 93-100.

Fincham AG & Belcourt AB. (1985) Amelogenin Biochemistry; current concepts, in, Butler WT (Ed.), The Chemistry and Biology of Mineralized Tissues, EBSCO Media Inc, Birmingham, Alabama. pp. 239-247.

Fincham AG, Aptel JD & Belcourt AB. (1985) The protein matrix of developing dental enamel, structure and function, in, Ruch JV & Belcourt AB (Eds.), Tooth Morphogenesis and Differentiation, INSERM Colloquium Series, Paris, 125, 315-332.

Belcourt AB, Fincham AG & Termine JD. (1983) Bovine high molecular weight amelogenin proteins, Calcified Tissue International, 35, 111-114.

Fincham AG, Belcourt AB, Termine JD, Butler WT & Cothran WC. (1983) Amelogenins; sequence homologies in enamel-matrix proteins from three mammalian species, Biochemical Journal, 211, 149-154.

Fincham AG, Belcourt AB & Termine JD. (1983) The molecular composition of the protein matrix of developing human dental enamel, Journal of Dental Research, 62, 11-15.

Fincham AG, Belcourt AB & Termine JD. (1983) Experimental approaches to the study of enamel matrix. in, Lazzari E (Ed.), CRC Handbook of Experimental Aspects of Oral Biology, CRC Press, Boca Raton, Florida. pp. 143-157.

Lyaruu DM, Termine JD, Belcourt AB & Fincham AG. (1982) Neonatal hamster molar tooth development; extraction and characterization of amelogenins, enamelins and soluble dentin proteins, Calcified Tissue International, 34, 86-96.

Belcourt AB, Fincham AG & Termine JD. (1982) Acid soluble bovine fetal enamelins, Journal of Dental Research, 61, 1031-1032.

Fincham AG, Belcourt AB, Lyaruu DM & Termine JD. (1982) Comparative protein biochemistry of developing dental enamel matrix from five mammalian species, Calcified Tissue International, 34, 182-189.

BelcourtAB, Fincham AG & Termine JD. (1982) Ethylenediamine-tetraacetic acid insoluble protein of adult human enamel, Caries Research, 16, 72-76.

Fincham AG, Belcourt AB & Termine JD. (1982) Changing patterns of enamel matrix proteins in the developing bovine tooth, Caries Research, 16, 64-71.

Fincham AG. (1982) The extracellular protein matrix of developing dental enamel, in, Anderson WA & Sadler W (Eds.), Perspectives in Differentiation and Hypertrophy, Elsevier, New York. pp. 135-156.

Fincham AG, Belcourt AB & Termine JD. (1981) The molecular composition of bovine fetal enamel matrix, in, Veis A (Ed.), Chemistry and Biology of Mineralized Tissues, Elsevier-North Holland Inc, New York. pp. 523-529.

Fincham AG, Belcourt AB, Termine JD, Butler WT & Cothran WC. (1981) Dental enamel matrix; sequences of two amelogenin polypeptides, Bioscience Reports, 1, 771-778.

Fincham AG. (1980) Changing amino acid profiles of developing dental enamel in individual human teeth and the comparison of the protein matrix of human and bovine enamel, Archives of oral Biology, 25, 669-674.

Fincham AG. (1979) Dental enamel matrix - isolation and partial sequence of a peptide component, Journal of Dental Research, 58B, 997-999.

Fincham AG. (1979) The amelogenin problem; a comparison of purified enamel matrix proteins, Calcified Tissue International, 26, 65-73.

Fincham AG. (1979) A simple procedure for the isolation of a major amelogenin polypeptide, Biochemical Journal, 181, 171-175.

Fincham AG, Burkland G & Shapiro IM (1972) Lipophilia of enamel matrix; a chemical investigation of the neutral lipids and lipophilic proteins of bovine foetal enamel matrix, Calcified Tissue Research, 9, 247-259. 339.

Fincham AG. (1968) Electrophoretic and Sephadex gel filtration studies of bovine foetal enamel matrix at acid pH, Calcified Tissue Research, 2, 353-357.